Rabu, 15 Juni 2011

DEFINITION OF ELECTRON MICROSCOPE (TEM AND SEM)

There are two main types of electron microscopes. The most widely used

electron microscope is the TEM (transmission electron microscope)

and the other is the SEM (scanning electron microscope).

In an electron microscope a ray of electrons is passed

from a cathode heated by an electric current toward the sample.

The higher the electric voltage, the greater the resolution.

The SEM differs from the TEM in that the SEM primarily

uses the same technology as television. The resolution is less

than that of the TEM but the focus area of the TEM is much

larger than that of the SEM.

One of the main drawbacks of both the TEM and SEM

is their inability to scan and analyze living material. Also, it is

necessary for most material to be saturated with heavy metals

that enable better contrast in both microscopes.

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